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The automated procedure for the determination of Ammonia utilizes the Berthelot reaction, in which the formation of a blue colored compound ( indophenol) occurs when l’ammonia reacts with sodium phenate followed by the reaction with sodium hypochlorite. Optimal PH range is 10.2-10.6 Sodium citrate is added to eliminate the precipitation of the hydroxide of calcium and magnesium The colored compound is measured at 660 nm
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